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Isolation of IL–12p70-competent human monocyte-derived dendritic cells.
Isolation of IL-12p70-competent human monocyte-derived dendritic cells.
Posted by Curtis

The methodology varies from experimental immunological studies to immunotherapy involves the application of dendritic cells originating from human monocyts (modcs). Variations in the dependent donor are quite large in the production of the MODC IL-12P70 affect the results of this methodology. It has been shown that modcs generated in standard conditions develop into two subsets based on CD1A expression with CD1A + modcs to become the main IL-12P70 manufacturer. But this has not been accepted in general, which we show here because the subset described as CD1A-negative does not reveal CD1A, but at a lower level of other subsets.

We then characterize the phenotype of the two sets of this section, indicating that the CD1A-hi subset has greater immunogenic phenotypes, making this subset more suitable for immunotherapy. The two sets of previous parts have been separated by sorting cells, but because this technique is not available for many laboratories and has incompatibility with clinical settings, more used techniques are guaranteed. Therefore we are tested if cell sorter activated magnetic is useful for this purpose, and shows that it is possible to isolate the CD1A-HI modCs that are competent IL-12P70 with purity <92%, regardless of the initial purity.

Hemohim improves the persistent down rules of immune response such as TH1 in irradiation mice γ fractionation by modulating <em> il </ em> – <amp> 12p70 </ em> signaling paths –stat4.

The mice irradiated throughout the body seem to experience Down rules of immune response T (Th) 1 – such as, and maintain persistent immunological imbalances. In this current study, we evaluate the influence of Hemohim (herbal products made from Angelica Radix, Cnidium Officinale, and Paeonia Japonica which are cultivated in Korea) to improve immunological imbalances caused by irradiation mice. Mice are exposed to rays twice a week (0.5 gy fraction) for a total dose of 5 gy, and hemohim is administered orally from 1 week before the first irradiation up to 1 week before the final analysis. All experiments were carried out 4 and 6 months after their first exposure. Hemohim improves the response of immune related Th1 and Th2 usually occurs in irradiation mice with or without immunization hemocyanin limpet dinitrofenylated.

Hemohim also restored natural killer cell activities without changing the percentage of natural killer cells in irradiation mice. Furthermore, the Hemohim administration prevents the reduction in the level of interleukin-12p70 in irradiation mice. Finally, we found that hemohim increases the phosphorylation of signal transducers and transcription activators (stat) 4 reduced in irradiation mice. Our findings show that Hemohim improves continuous down rules from immune responses like them by modulating the IL-12P70 / Pstat4 signaling path.

Plasma Neonatal Polarized Cytokine Responses mediated by TLR4 towards Low <em> il </ em> 12p70 </ em> and height <em> il </ em> -10 production through different factors.

Human neonates are very susceptible to infection, which will mostly be caused by disruption of the default immune function. Receptor responts such as neonatal toll road (TLR) bias against the generation of pro-inflammatory polarization cytokines / TH1, but the underlying mechanism is incomplete defined. Here, we show that the neonatal plasma is polarized the production of cytokines mediated by TLR4. When exposed to cord blood plasma, mononuclear cells (MCS) produce IL-12P70 mediated by TLR4 significantly and IL-10 higher than the MC exposed to adult plasma. Oppression by neonatal plasma production IL-12P70 mediated TLR4, but not induction of IL-10 production mediated by TLR4, maintained to the age of 1 month.

Plasma blood rope blood provides a similar pattern of the MC cytokine response to TLR3 and TLR8 agonists, showing activity against MYD88 agonists depending on and MYD88-independent. The factor that causes an increase in IL-10 production mediated by TLR4 by plasma blood cables is hot labil, lost after the thinning of protein and independent of the lipoprotein binding protein (LBP) or CD14 (SCD14) dissolves. The factor that causes the inhibition of production of IL-12P70 mediated by TLR4 with blood plasma rope resistant to heat inactivation or depletion of proteins and does not depend on IL-10, vitamin D and prostaglandin e2. In conclusion, neonatal plasma humans contain at least two different factors that suppress the production of IL-12P70 mediated by TLR4 or inducing IL-10 or production. Further identification of these factors will provide insight into ontogeny from default immune development and can identify new targets for prevention and treatment of neonatal infections.

Isolation of <em>IL</em>-<em>12p70</em>-competent human monocyte-derived dendritic cells.

The preparation of the OK432 bacteria induces <em> il </ em> – <em> 12p70 </ em> secretion in human dendritic cells in a way that depends on TLR3.

Dendritic cells (DC) used in immunotherapy therapeutic cancers must be able to stimulate T cells which result in immune responses that can efficiently target cancer cells. One critical obstacle is the lack of production of IL-12P70 when bringing DC, which is repaired using the preparation of the OK432 bacteria (trade name of the Picibanil) to cut the cell. To identify the mechanism behind DC’s OK432 stimulation, we investigate different TLR contributions to check their involvement in the production of IL-12P70. By combining different inhibitors from TLR signaling, we show here that TLR3 is responsible for the production of IL-12P70 DC which is induced by OK432.

Human IL-12p70 ELISA Kit
E28L0027 96T
EUR 666.67

Human IL-12p70 ELISA Kit
M950070096 1 unit
EUR 411.32

Human IL-12p70 ELISA Kit
MBS335445-10x96WellsPrecoatedplate 10x96Wells(Pre-coated-plate)
EUR 2465

Human IL-12p70 ELISA Kit
MBS335445-1x96WellsPrecoatedplate 1x96Wells(Pre-coated-plate)
EUR 375

Human IL-12p70 ELISA Kit
MBS335445-2x96WellsPrecoatedplate 2x96Wells(Pre-coated-plate)
EUR 485

Human IL-12p70 ELISA Kit
MBS8579151-48Tests 48Tests
EUR 390

Human IL-12p70 ELISA Kit
MBS8579151-5x96Tests 5x96Tests
EUR 2275

Human IL-12p70 ELISA Kit
MBS8579151-96Tests 96Tests
EUR 495

Human IL-12p70 ELISA KIT
MBS8506087-48Wells 48Wells
EUR 390

Human IL-12p70 ELISA KIT
MBS8506087-5x96Wells 5x96Wells
EUR 2275

Human IL-12p70 ELISA KIT
MBS8506087-96Wells 96Wells
EUR 495

Human IL-12p70 ELISA KIT
MBS9424804-5x96Tests 5x96Tests
EUR 2225

Human IL-12p70 ELISA KIT
MBS9424804-96Tests 96Tests
EUR 485

Human IL-12p70 Uncoated ELISA Kit
RK04532 10Plates
EUR 584.51

Human IL-12p70 ELISA kit (Quick Test)
E22-HC010QT.48 48T
EUR 495

Human IL-12p70 ELISA kit (Quick Test)
E22-HC010QT.96 96T
EUR 595

Human IL-12p70 ELISPOT kit
CT240-PB2 2-plate
EUR 498

Human IL-12p70 ELISPOT kit
CT240-PB5 5-plate
EUR 681.6

Human IL-12p70 ELISPOT kit
CT240-PR2 2-plate
EUR 498

Human IL-12p70 ELISPOT kit
CT240-PR5 5-plate
EUR 667.2

Human IL-12p70 ELISPOT kit
CT240-T2 2-plate
EUR 432

Human IL-12p70 ELISPOT kit
CT240-T5 5-plate
EUR 694.8

Human  IL-12p70 ELISA kit (High Sensitivity)
E22-HC010(H).48 48T
EUR 495
Description: 1.5pg/ml

Human  IL-12p70 ELISA kit (High Sensitivity)
E22-HC010(H).96 96T
EUR 595
Description: 1.5pg/ml

Human IL-12p70 High Sensitivity ELISA Kit
E28L0679 96T
EUR 952.38

Human IL-12p70 ELISA Kit (High Sensitivity)
MBS8579150-48Tests 48Tests
EUR 595

Human IL-12p70 ELISA Kit (High Sensitivity)
MBS8579150-5x96Tests 5x96Tests
EUR 3190

Human IL-12p70 ELISA Kit (High Sensitivity)
MBS8579150-96Tests 96Tests
EUR 695

Human IL-12p70
MBS692496-0002mg 0.002mg
EUR 300

Human IL-12p70
MBS692496-001mg 0.01mg
EUR 450

Human IL-12p70
MBS692496-5x001mg 5x0.01mg
EUR 1725

Sunlong Medical™ Human IL-12p70 ELISA Kit
EL0248Hu-48T 48T
EUR 398

Sunlong Medical™ Human IL-12p70 ELISA Kit
EL0248Hu-96T 96T
EUR 655

Human IL-12p70 ELISA antibody pair
CT750-10 10-plate
EUR 656.4

Human IL-12p70 ELISA antibody pair
CT750-20 20-plate
EUR 1118.4

Rat IL-12P70 ELISA Kit
DEIA1175 96T
EUR 709.8
Description: cell culture supernatant, serum, plasma, tissues

Mouse IL-12p70 ELISA kit
E22-MC006.48 48T
EUR 295
Description: 8pg/ml

Mouse IL-12p70 ELISA kit
E22-MC006.96 96T
EUR 395
Description: 8pg/ml

Mouse IL-12p70 ELISA Kit
E16ME0013 96T
EUR 833.33

Mouse IL-12p70 ELISA Kit
E28L0063 96T
EUR 666.67

Mouse IL-12p70 ELISA Kit
MBS8579363-48Tests 48Tests
EUR 390

Mouse IL-12p70 ELISA Kit
MBS8579363-5x96Tests 5x96Tests
EUR 2275

Mouse IL-12p70 ELISA Kit
MBS8579363-96Tests 96Tests
EUR 495

Mouse IL-12p70 ELISA KIT
MBS8506065-48Wells 48Wells
EUR 390

Mouse IL-12p70 ELISA KIT
MBS8506065-5x96Wells 5x96Wells
EUR 2275

Mouse IL-12p70 ELISA KIT
MBS8506065-96Wells 96Wells
EUR 495

Human IL-12/IL-23 p40 ELISA Kit
CEA-C203 96tests
EUR 545
Description: ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IL-12/IL-23 p40 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates. A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS. ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

Human IL-12/IL-23 p40 ELISA Kit
CEA-C203-96tests 96tests
EUR 545
Description: ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IL-12/IL-23 p40 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates. A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS. ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

Human IL-12/IL-23 p40 ELISA Kit
E28L0593 96T
EUR 666.67

Human IL-12/IL-23 p40 ELISA Kit
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EUR 2615

Human IL-12/IL-23 p40 ELISA Kit
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EUR 570

Human IL-12/IL-23 p40 ELISA Kit
RK00013 96 Tests
EUR 322.37

Sunlong Medical™ Human IL-12p70 High Sensitivity ELISA Kit
HS-EL0238Hu-48T 48T
EUR 455

Sunlong Medical™ Human IL-12p70 High Sensitivity ELISA Kit
HS-EL0238Hu-96T 96T Ask for price

Human IL-12 p70 ELISA Kit
CEK1741 96T
EUR 538
Description: Sandwich ELKSA for quantitative detection of Human IL-12 p70 concentrations.

Human IL-12 p40 ELISA Kit
CEK1742 96T
EUR 538
Description: Sandwich ELKSA for quantitative detection of Human IL-12 p40 concentrations.

Human IL-12(p70) ELISA Kit
GWB-ZZD009 1x96 well plate Ask for price

Human IL-12(p40) ELISA Kit
GWB-ZZD010 1x96 well plate Ask for price

Human IL-12 (p40) ELISA KIT
E42EH-204 96T/48T
EUR 795

Human IL-27 (p28) ELISA KIT
E42EH-213 96T/48T
EUR 795

Human IL-12/P70 ELISA Kit
EHI0362 96Tests
EUR 625.2

IL-12 p40 ELISA KIT|Human
EF000144 96 Tests
EUR 826.8

IL-12 p35 ELISA KIT|Human
EF007351 96 Tests
EUR 826.8

Human IL-12(p70) ELISA kit
LF-EK50181 1×96T
EUR 777.6

Human IL-12 p40 ELISA Kit
MBS824762-5x96Tests 5x96Tests
EUR 2755

Human IL-12 p40 ELISA Kit
MBS824762-96Tests 96Tests
EUR 620

Human IL-12 p70 ELISA Kit
MBS824795-5x96Tests 5x96Tests
EUR 2755

Human IL-12 p70 ELISA Kit
MBS824795-96Tests 96Tests
EUR 620

Human IL-12 p40 ELISA Kit
MBS824673-5x96Tests 5x96Tests
EUR 2755

Human IL-12 p40 ELISA Kit
MBS824673-96Tests 96Tests
EUR 620

Human IL-12 p40 ELISA Kit
MBS8244570-5x96Tests 5x96Tests
EUR 2755

Human IL-12 p40 ELISA Kit
MBS8244570-96Tests 96Tests
EUR 620

Human IL-12 p70 ELISA Kit
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Human IL-12 p70 ELISA Kit
MBS8244589-96Tests 96Tests
EUR 620

Human IL-12 p70 ELISA Kit
MBS824473-5x96Tests 5x96Tests
EUR 2755

Human IL-12 p70 ELISA Kit
MBS824473-96Tests 96Tests
EUR 620

Human IL-12 p70 ELISA Kit
MBS9719276-48Tests 48Tests
EUR 300

Human IL-12 p70 ELISA Kit
MBS9719276-5x48Tests 5x48Tests
EUR 1365

Human IL-12 p70 ELISA Kit
MBS9719276-5x96Tests 5x96Tests
EUR 1750

Human IL-12 p70 ELISA Kit
MBS9719276-96Tests 96Tests
EUR 385

Human IL-12 p70 ELISA Kit
MBS9135734-5x96Tests 5x96Tests
EUR 2615

Human IL-12 p70 ELISA Kit
MBS9135734-96Tests 96Tests
EUR 570

Human IL-12 p70 ELISA Kit
RK00014 96 Tests
EUR 322.37

Human IL-12p40 + p70 ELISA Kit
MBS335453-10x96WellsPrecoatedplate 10x96Wells(Pre-coated-plate)
EUR 2465

Human IL-12p40 + p70 ELISA Kit
MBS335453-1x96WellsPrecoatedplate 1x96Wells(Pre-coated-plate)
EUR 375

Human IL-12p40 + p70 ELISA Kit
MBS335453-2x96WellsPrecoatedplate 2x96Wells(Pre-coated-plate)
EUR 485

Human IL-12p70 ELISPOT antibody pair
CT650-10 10-plate
EUR 633.6

Human IL-12p70 ELISPOT antibody pair
CT650-20 20-plate
EUR 1107.6

IL-12 P40 Human ELISA Kit
MBS355454-5x96Tests 5x96Tests
EUR 2190

IL-12 P40 Human ELISA Kit
MBS355454-96Tests 96Tests
EUR 485

IL-12 P70 Human ELISA Kit
MBS355278-5x96Tests 5x96Tests
EUR 2190

IL-12 P70 Human ELISA Kit
MBS355278-96Tests 96Tests
EUR 485

Human IL-12/23 P40 ELISA kit
E22-HC152.48 48T
EUR 295
Description: 8pg/ml

Human IL-12/23 P40 ELISA kit
E22-HC152.96 96T
EUR 395
Description: 8pg/ml

Human IL-12/23 P40 ELISA Kit
MBS8579218-48Tests 48Tests
EUR 390

Human IL-12/23 P40 ELISA Kit
MBS8579218-5x96Tests 5x96Tests
EUR 2275

Human IL-12/23 P40 ELISA Kit
MBS8579218-96Tests 96Tests
EUR 495

Human IL-12(p70) ELISA kit (4X96T)
LF-EK50182 4×96T
EUR 2641.2

CymaxTM Human IL-12 (p70) ELISA Kit
LF-EK0264 1×96T
EUR 477.6

Mouse Interleukin 12 (IL-12P70) ELISA Kit
MBS3805272-10x96StripWells 10x96-Strip-Wells
EUR 6725

Mouse Interleukin 12 (IL-12P70) ELISA Kit
MBS3805272-48StripWells 48-Strip-Wells
EUR 550

Mouse Interleukin 12 (IL-12P70) ELISA Kit
MBS3805272-5x96StripWells 5x96-Strip-Wells
EUR 3420

Mouse Interleukin 12 (IL-12P70) ELISA Kit
MBS3805272-96StripWells 96-Strip-Wells
EUR 765

Mouse IL-12p70 High Sensitivity ELISA Kit
E28L0749 96T
EUR 1003.17

IL-12 (p70) ELISA Kit (Human) (OKBB00196)
OKBB00196 96 Tests
EUR 606
Description: Description of target: Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response.i And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation.ii Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.iii The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75 KDa.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 2 pg/ml

IL-12(p40) ELISA Kit (Human) (OKBB00198)
OKBB00198 96 Tests
EUR 606
Description: Description of target: Interleukin-12 (IL12; formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits.1 The larger 40-kDa subunit (p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit (p35) is related to IL6 and GCSF.2 Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p70 heterodimer.3 Interleukin (IL)-12 has been cloned on the basis of its ability to activate natural killer (NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon (IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection; for the development of a Th1 response, critical for effective host defense against intracellular pathogens; and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype.4 The subunits map to different chromosomes: p40 (IL12B) to 5q31-q33 and p35 (IL12A) to 3p12-3q13.2.2 The standard product used in this kit is recombinant human IL-12(p40), which is a 40KDa glycoprotein consisting of 306 amino acids.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 2 pg/ml

Mouse IL-12p70 ELISA KTI
E16ME0013-048 48 wells
EUR 295

Mouse IL-12p70 ELISA KTI
E16ME0013-096 96 wells
EUR 395

ELISA kit for Human IL-12 p70
KET6020-48T 48T
EUR 242.4
Description: Quantitative sandwich ELISA for measuring Human IL-12 p70 in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human IL-12 p70
KET6020-5platesof96wells 5 plates of 96 wells
EUR 1292.4
Description: Quantitative sandwich ELISA for measuring Human IL-12 p70 in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human IL-12 p70
KET6020-96T 96T
EUR 346.8
Description: Quantitative sandwich ELISA for measuring Human IL-12 p70 in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human IL-12 p70 FAST ELISA Kit
RK04448 96T
EUR 322.37

Human IL-12p40 (Interleukin 12p40) Pre-Coated ELISA Kit
MBS669155-5x96Tests 5x96Tests
EUR 3545

Human IL-12p40 (Interleukin 12p40) Pre-Coated ELISA Kit
MBS669155-96Tests 96Tests
EUR 770

Nori® Human IL-12 p35 ELISA Kit
GR111522 96-well
EUR 461

Nori® Human IL-12 p40 ELISA Kit
GR111523 96-well
EUR 461

Sunlong Medical™ Human IL-12/IL-23 p40 ELISA Kit
EL0185Hu-48T 48T
EUR 398

Sunlong Medical™ Human IL-12/IL-23 p40 ELISA Kit
EL0185Hu-96T 96T
EUR 569

Human Interleukin 12 P70 (IL-12 p70) ELISA Kit
abx251064-96tests 96 tests
EUR 764.4

Human Interleukin 12 P70,IL-12;P70 ELISA KIT
E0099Hu-1096T 10*96T
EUR 4122

Human Interleukin 12 P70,IL-12;P70 ELISA KIT
E0099Hu-48wells 48 wells
EUR 300

Human Interleukin 12 P70,IL-12;P70 ELISA KIT
E0099Hu-596T 5*96T
EUR 2061

Human Interleukin 12 P70,IL-12;P70 ELISA KIT
E0099Hu-96wells 96 wells
EUR 458

Human Interleukin 12 P40,IL-12;P40 ELISA KIT
E0100Hu-1096T 10*96T
EUR 4122

Human Interleukin 12 P40,IL-12;P40 ELISA KIT
E0100Hu-48wells 48 wells
EUR 300

Human Interleukin 12 P40,IL-12;P40 ELISA KIT
E0100Hu-596T 5*96T
EUR 2061

Human Interleukin 12 P40,IL-12;P40 ELISA KIT
E0100Hu-96wells 96 wells
EUR 458

Human Interleukin 27 p28,IL-27 p28 ELISA KIT
JOT-EK7048Hu 96 wells Ask for price
Description: Human

Human Interleukin 12 P70,IL-12;P70 ELISA KIT
JOT-EK0099Hu 96 wells Ask for price
Description: Human

Human Interleukin 12 P40,IL-12;P40 ELISA KIT
JOT-EK0100Hu 96 wells Ask for price
Description: Human

Human IL-12(p40)(Interleukin 12 p40) ELISA Kit
E39EH0175 96T
EUR 595

Human IL-12 p35 (Interleukin 12 p35) ELISA Kit
EKE60990-5x96T 5x96T
EUR 3124.08

Human IL-12 p35 (Interleukin 12 p35) ELISA Kit
EKE60990-96T 96T
EUR 657.7

In addition, our data shows that ligands that trigger the secretion of IL-12P70 on TLR3 stimulation are sensitive to proteinase and some are also RNASE care. The fact that bacterial compounds such as OK432 can activate the TLR3 path in human DC are new findings. OK432 shows an important ability to induce IL-12P70 production, which is very relevant in DC-based cancer immunotherapy.

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