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Isolation of IL12p70-competent human monocyte-derived dendritic cells.

Isolation of IL-12p70-competent human monocyte-derived dendritic cells.
Posted by Curtis

The methodology varies from experimental immunological studies to immunotherapy involves the application of dendritic cells originating from human monocyts (modcs). Variations in the dependent donor are quite large in the production of the MODC IL-12P70 affect the results of this methodology. It has been shown that modcs generated in standard conditions develop into two subsets based on CD1A expression with CD1A + modcs to become the main IL-12P70 manufacturer. But this has not been accepted in general, which we show here because the subset described as CD1A-negative does not reveal CD1A, but at a lower level of other subsets.

We then characterize the phenotype of the two sets of this section, indicating that the CD1A-hi subset has greater immunogenic phenotypes, making this subset more suitable for immunotherapy. The two sets of previous parts have been separated by sorting cells, but because this technique is not available for many laboratories and has incompatibility with clinical settings, more used techniques are guaranteed. Therefore we are tested if cell sorter activated magnetic is useful for this purpose, and shows that it is possible to isolate the CD1A-HI modCs that are competent IL-12P70 with purity <92%, regardless of the initial purity.

Hemohim improves the persistent down rules of immune response such as TH1 in irradiation mice γ fractionation by modulating <em> il </ em> – <amp> 12p70 </ em> signaling paths –stat4.

The mice irradiated throughout the body seem to experience Down rules of immune response T (Th) 1 – such as, and maintain persistent immunological imbalances. In this current study, we evaluate the influence of Hemohim (herbal products made from Angelica Radix, Cnidium Officinale, and Paeonia Japonica which are cultivated in Korea) to improve immunological imbalances caused by irradiation mice. Mice are exposed to rays twice a week (0.5 gy fraction) for a total dose of 5 gy, and hemohim is administered orally from 1 week before the first irradiation up to 1 week before the final analysis. All experiments were carried out 4 and 6 months after their first exposure. Hemohim improves the response of immune related Th1 and Th2 usually occurs in irradiation mice with or without immunization hemocyanin limpet dinitrofenylated.

Hemohim also restored natural killer cell activities without changing the percentage of natural killer cells in irradiation mice. Furthermore, the Hemohim administration prevents the reduction in the level of interleukin-12p70 in irradiation mice. Finally, we found that hemohim increases the phosphorylation of signal transducers and transcription activators (stat) 4 reduced in irradiation mice. Our findings show that Hemohim improves continuous down rules from immune responses like them by modulating the IL-12P70 / Pstat4 signaling path.

Plasma Neonatal Polarized Cytokine Responses mediated by TLR4 towards Low <em> il </ em> 12p70 </ em> and height <em> il </ em> -10 production through different factors.

Human neonates are very susceptible to infection, which will mostly be caused by disruption of the default immune function. Receptor responts such as neonatal toll road (TLR) bias against the generation of pro-inflammatory polarization cytokines / TH1, but the underlying mechanism is incomplete defined. Here, we show that the neonatal plasma is polarized the production of cytokines mediated by TLR4. When exposed to cord blood plasma, mononuclear cells (MCS) produce IL-12P70 mediated by TLR4 significantly and IL-10 higher than the MC exposed to adult plasma. Oppression by neonatal plasma production IL-12P70 mediated TLR4, but not induction of IL-10 production mediated by TLR4, maintained to the age of 1 month.

Plasma blood rope blood provides a similar pattern of the MC cytokine response to TLR3 and TLR8 agonists, showing activity against MYD88 agonists depending on and MYD88-independent. The factor that causes an increase in IL-10 production mediated by TLR4 by plasma blood cables is hot labil, lost after the thinning of protein and independent of the lipoprotein binding protein (LBP) or CD14 (SCD14) dissolves. The factor that causes the inhibition of production of IL-12P70 mediated by TLR4 with blood plasma rope resistant to heat inactivation or depletion of proteins and does not depend on IL-10, vitamin D and prostaglandin e2. In conclusion, neonatal plasma humans contain at least two different factors that suppress the production of IL-12P70 mediated by TLR4 or inducing IL-10 or production. Further identification of these factors will provide insight into ontogeny from default immune development and can identify new targets for prevention and treatment of neonatal infections.

Isolation of <em>IL</em>-<em>12p70</em>-competent human monocyte-derived dendritic cells.

The preparation of the OK432 bacteria induces <em> il </ em> – <em> 12p70 </ em> secretion in human dendritic cells in a way that depends on TLR3.

Dendritic cells (DC) used in immunotherapy therapeutic cancers must be able to stimulate T cells which result in immune responses that can efficiently target cancer cells. One critical obstacle is the lack of production of IL-12P70 when bringing DC, which is repaired using the preparation of the OK432 bacteria (trade name of the Picibanil) to cut the cell. To identify the mechanism behind DC’s OK432 stimulation, we investigate different TLR contributions to check their involvement in the production of IL-12P70. By combining different inhibitors from TLR signaling, we show here that TLR3 is responsible for the production of IL-12P70 DC which is induced by OK432.

Human IL-12p70 ELISA Kit

CRS-B009 96tests
EUR 2696.4
Description: ClinMaxTM ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IL-12p70 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates. A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS. ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

Human IL-12p70 ELISA Kit

M950070096 1 unit
EUR 411.32

Human IL-12p70 Uncoated ELISA Kit

RK04532 10Plates
EUR 280

Human  IL-12p70 ELISA kit (High Sensitivity)

E22-HC010(H).48 48T
EUR 495

Human  IL-12p70 ELISA kit (High Sensitivity)

E22-HC010(H).96 96T
EUR 595

Human IL-12p70 High Sensitivity ELISA Kit

E28L0679 96T
EUR 952.38

Human IL-12p70 ELISPOT kit

CT240-PB2 2-plate
EUR 498

Human IL-12p70 ELISPOT kit

CT240-PB5 5-plate
EUR 681.6

Human IL-12p70 ELISPOT kit

CT240-PR2 2-plate
EUR 498

Human IL-12p70 ELISPOT kit

CT240-PR5 5-plate
EUR 667.2

Human IL-12p70 ELISPOT kit

CT240-T2 2-plate
EUR 432

Human IL-12p70 ELISPOT kit

CT240-T5 5-plate
EUR 694.8

Rat IL-12P70 ELISA Kit

DEIA1175 96T
EUR 1132.8
Description: The Rat IL-12P70 ELISA kit is designed to detect and quantify the level of Rat IL-12P70 in cell culture supernatant, serum, plasma and tissue. This kit is for research use only and not intended for diagnostic purposes.

Mouse IL-12p70 ELISA Kit

E16ME0013 96T
EUR 833.33

Mouse IL-12p70 ELISA kit

E22-MC006.48 48T
EUR 295

Mouse IL-12p70 ELISA kit

E22-MC006.96 96T
EUR 395

Mouse IL-12p70 ELISA Kit

E28L0063 96T
EUR 666.67

Human IL-12p70 ELISA antibody pair

CT750-10 10-plate
EUR 656.4

Human IL-12p70 ELISA antibody pair

CT750-20 20-plate
EUR 1118.4

Human IL-12/IL-23 p40 ELISA Kit

E28L0593 96T
EUR 666.67

Human IL-12/IL-23 p40 ELISA Kit

RK00013 96 Tests
EUR 280

IL-12 p40 ELISA KIT|Human

EF000144 96 Tests
EUR 826.8

IL-12 p35 ELISA KIT|Human

EF007351 96 Tests
EUR 826.8

Human IL-12 (p40) ELISA KIT

E42EH-204 96T/48T Ask for price

Human IL-27 (p28) ELISA KIT

E42EH-213 96T/48T Ask for price

Human IL-12/P70 ELISA Kit

EHI0362 96Tests
EUR 625.2

Human IL-12(p70) ELISA kit

LF-EK50181 1×96T
EUR 777.6

Human IL-12 p70 ELISA Kit

RK00014 96 Tests
EUR 280

Human IL-12(p70) ELISA Kit

GWB-ZZD009 1x96 well plate Ask for price

Human IL-12(p40) ELISA Kit

GWB-ZZD010 1x96 well plate Ask for price

Mouse IL-12p70 High Sensitivity ELISA Kit

E28L0749 96T
EUR 1003.17

Mouse IL-12p70 ELISA KTI

E16ME0013-048 48 wells
EUR 295

Mouse IL-12p70 ELISA KTI

E16ME0013-096 96 wells
EUR 395

Human IL-12/23 P40 ELISA kit

E22-HC152.48 48T
EUR 295

Human IL-12/23 P40 ELISA kit

E22-HC152.96 96T
EUR 395

Human IL-12(p70) ELISA kit (4X96T)

LF-EK50182 4×96T
EUR 2641.2

Human IL-12p70 ELISPOT antibody pair

CT650-10 10-plate
EUR 633.6

Human IL-12p70 ELISPOT antibody pair

CT650-20 20-plate
EUR 1107.6

CymaxTM Human IL-12 (p70) ELISA Kit

LF-EK0264 1×96T
EUR 477.6

IL-12 (p70) ELISA Kit (Human) (OKBB00196)

OKBB00196 96 Tests
EUR 606
Description: Description of target: Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response.i And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation.ii Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.iii The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75 KDa.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 2 pg/ml

IL-12(p40) ELISA Kit (Human) (OKBB00198)

OKBB00198 96 Tests
EUR 606
Description: Description of target: Interleukin-12 (IL12; formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits.1 The larger 40-kDa subunit (p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit (p35) is related to IL6 and GCSF.2 Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p70 heterodimer.3 Interleukin (IL)-12 has been cloned on the basis of its ability to activate natural killer (NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon (IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection; for the development of a Th1 response, critical for effective host defense against intracellular pathogens; and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype.4 The subunits map to different chromosomes: p40 (IL12B) to 5q31-q33 and p35 (IL12A) to 3p12-3q13.2.2 The standard product used in this kit is recombinant human IL-12(p40), which is a 40KDa glycoprotein consisting of 306 amino acids.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 2 pg/ml

ELISA kit for Human IL-12 p70

KET6020-48T 48T
EUR 242.4
Description: Quantitative sandwich ELISA for measuring Human IL-12 p70 in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human IL-12 p70

KET6020-5platesof96wells 5 plates of 96 wells
EUR 1292.4
Description: Quantitative sandwich ELISA for measuring Human IL-12 p70 in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human IL-12 p70

KET6020-96T 96T
EUR 346.8
Description: Quantitative sandwich ELISA for measuring Human IL-12 p70 in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human IL-12 p70 FAST ELISA Kit

RK04448 96T
EUR 280

Nori® Human IL-12 p35 ELISA Kit

GR111522 96-well
EUR 461

Nori® Human IL-12 p40 ELISA Kit

GR111523 96-well
EUR 461

OKRC01051-96W - IL-12p70 ELISA Kit (Rhesus Macaque)

OKRC01051-96W 96Wells
EUR 525

Human IL-12(p40)(Interleukin 12 p40) ELISA Kit

EH0175 96T
EUR 571.5
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Human IL-12(p35)(Interleukin 12 p35) ELISA Kit

EKF58599-48T 48T
EUR 396.9

Human IL-12(p35)(Interleukin 12 p35) ELISA Kit

EKF58599-5x96T 5x96T
EUR 2693.25

Human IL-12(p35)(Interleukin 12 p35) ELISA Kit

EKF58599-96T 96T
EUR 567

Human IL-12(p40)(Interleukin 12 p40) ELISA Kit

EKF57060-48T 48T
EUR 396.9

Human IL-12(p40)(Interleukin 12 p40) ELISA Kit

EKF57060-5x96T 5x96T
EUR 2693.25

Human IL-12(p40)(Interleukin 12 p40) ELISA Kit

EKF57060-96T 96T
EUR 567

Human IL-12 p35 (Interleukin 12 p35) ELISA Kit

EKE60990-5x96T 5x96T
EUR 3124.08

Human IL-12 p35 (Interleukin 12 p35) ELISA Kit

EKE60990-96T 96T
EUR 657.7

Human IL-12 p40 (Interleukin 12 p40) ELISA Kit

EKE60132-5x96T 5x96T
EUR 2667.6

Human IL-12 p40 (Interleukin 12 p40) ELISA Kit

EKE60132-96T 96T
EUR 561.6

Human IL-12(p40)(Interleukin 12 p40) ELISA Kit

E39EH0175 96T
EUR 595

Human IL-12(p35)(Interleukin 12 p35) ELISA Kit

EH4146 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Human Interleukin 12 P70 (IL-12 p70) ELISA Kit

abx251064-96tests 96 tests
EUR 764.4

Human IL-12 p40(Interleukin 12 p40) ELISA Kit

RE1076H-48wells 48 wells
EUR 116.55

Human IL-12 p40(Interleukin 12 p40) ELISA Kit

RE1076H-96wells 96 wells
EUR 161.55

Human IL-12 p35(Interleukin 12 p35) ELISA Kit

RE1957H-48wells 48 wells
EUR 125.55

Human IL-12 p35(Interleukin 12 p35) ELISA Kit

RE1957H-96wells 96 wells
EUR 179.55

Human interleukin 27 p28(IL-27 p28) ELISA Kit

NSL1540Hu 96 wells
EUR 468

Human Interleukin 12 p70(IL-12 p70)ELISA Kit

NSL1925Hu 96 wells
EUR 468

Human Interleukin 12 p40(IL-12 p40)ELISA Kit

NSL1926Hu 96 wells
EUR 468

EliKine™ Human IL-12 p70 ELISA Kit

KTE6020-48T 48 T
EUR 189
Description: EliKine™ Human IL-12 p70 ELISA Kit employs a two-site sandwich ELISA to quantitate Human IL-12 p70.

EliKine™ Human IL-12 p70 ELISA Kit

KTE6020-96T 96 T
EUR 289
Description: EliKine™ Human IL-12 p70 ELISA Kit employs a two-site sandwich ELISA to quantitate Human IL-12 p70.

Human IL-12 p35-Interleukin 12 p35- ELISA Kit

E-EL-H1647-24Tests 24 Tests
EUR 150
Description: Sandwich

Human IL-12 p35-Interleukin 12 p35- ELISA Kit

E-EL-H1647-48Tests 48 Tests
EUR 487
Description: Sandwich

Human IL-12 p35-Interleukin 12 p35- ELISA Kit

E-EL-H1647-96Tests 96 Tests
EUR 609
Description: Sandwich

Human IL-12 p35-Interleukin 12 p35- ELISA Kit

E-EL-H1647-96Tests10 96 Tests *10
EUR 6090
Description: Sandwich

Human IL-12 p35-Interleukin 12 p35- ELISA Kit

E-EL-H1647-96Tests5 96 Tests *5
EUR 3045
Description: Sandwich

Human Interleukin 12,IL-12/P70 ELISA KIT

201-12-0100 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Interleukin 12,IL-12/P40 ELISA KIT

201-12-0101 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Interleukin 12,IL-12/P70 ELISA kit

E01I0432-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Interleukin 12,IL-12/P70 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Interleukin 12,IL-12/P70 ELISA kit

E01I0432-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Interleukin 12,IL-12/P70 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Interleukin 12,IL-12/P70 ELISA kit

E01I0432-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Interleukin 12,IL-12/P70 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Interleukin 12(IL-12,P70) Elisa Kit

EK710274 96 Wells
EUR 0.77

Human Interleukin 12,IL-12/P40 ELISA KIT

EKC40069-48T 48T
EUR 412.02

Human Interleukin 12,IL-12/P40 ELISA KIT

EKC40069-5x96T 5x96T
EUR 2795.85

Human Interleukin 12,IL-12/P40 ELISA KIT

EKC40069-96T 96T
EUR 588.6

Human Interleukin 12,IL-12/P70 ELISA KIT

EKC40070-48T 48T
EUR 412.79

Human Interleukin 12,IL-12/P70 ELISA KIT

EKC40070-5x96T 5x96T
EUR 2801.08

Human Interleukin 12,IL-12/P70 ELISA KIT

EKC40070-96T 96T
EUR 589.7

Human Interleukin 12,IL-12/P70 ELISA KIT

EKC42809-48T 48T
EUR 427

Human Interleukin 12,IL-12/P70 ELISA KIT

EKC42809-5x96T 5x96T
EUR 2897.5

Human Interleukin 12,IL-12/P70 ELISA KIT

EKC42809-96T 96T
EUR 610

Human Interleukin 12(IL-12/P70)ELISA Kit

GA-E0139HM-48T 48T
EUR 346.8

Human Interleukin 12(IL-12/P70)ELISA Kit

GA-E0139HM-96T 96T
EUR 559.2

Human Interleukin 12(IL-12/P40)ELISA Kit

GA-E0140HM-48T 48T
EUR 346.8

Human Interleukin 12(IL-12/P40)ELISA Kit

GA-E0140HM-96T 96T
EUR 559.2

Human Interleukin 12, IL-12/P40 ELISA KIT

CSB-E04598h-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Interleukin 12, IL-12/P40 in samples from serum, plasma, cell culture supernates, urine, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Interleukin 12, IL-12/P40 ELISA KIT

1-CSB-E04598h
  • Ask for price
  • Ask for price
  • Ask for price
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Interleukin 12, IL-12/P40 in samples from serum, plasma, cell culture supernates, urine, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Interleukin 12,IL-12/P70 ELISA KIT

CSB-E04599h(1) 96Т
EUR 496
Description: serum, cell culture supernates, tissue homogenates

Human Interleukin 12, IL-12/P70 ELISA KIT

CSB-E04599h-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Interleukin 12, IL-12/P70 in samples from serum, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Interleukin 12, IL-12/P70 ELISA KIT

1-CSB-E04599h
  • Ask for price
  • Ask for price
  • Ask for price
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Interleukin 12, IL-12/P70 in samples from serum, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Interleukin 12,IL-12/P70 ELISA Kit

CSB-E04599h-IS 96Т
EUR 496
Description: serum, plasma, cell culture supernates

Human Interleukin 12,IL-12/P70 ELISA KIT

CN-03218H1 96T
EUR 525.6

Human Interleukin 12,IL-12/P70 ELISA KIT

CN-03218H2 48T
EUR 346.8

Human Interleukin 12,IL-12/P40 ELISA KIT

CN-03219H1 96T
EUR 525.6

Human Interleukin 12,IL-12/P40 ELISA KIT

CN-03219H2 48T
EUR 346.8

Human Interleukin 12(IL-12/P70)ELISA Kit

QY-E04328 96T
EUR 433.2

Human Interleukin 12(IL-12/P40)ELISA Kit

QY-E04329 96T
EUR 480

Human Interleukin 12,IL-12/P40 ELISA Kit

SL0971Hu 96 Tests
EUR 468

Human Interleukin 12,IL-12/P70 ELISA Kit

SL0972Hu 96 Tests
EUR 468

Human Interleukin 12,IL-12/P70 ELISA Kit

YLA1522HU-48T 48T Ask for price

Human Interleukin 12,IL-12/P70 ELISA Kit

YLA1522HU-96T 96T Ask for price

Human Interleukin 12,IL-12/P40 ELISA Kit

YLA1523HU-48T 48T Ask for price

Human Interleukin 12,IL-12/P40 ELISA Kit

YLA1523HU-96T 96T Ask for price

OKRC00565-96W - IL-12 p40 ELISA Kit (Human)

OKRC00565-96W 96Wells
EUR 500

OKRC00566-96W - IL-12 p70 ELISA Kit (Human)

OKRC00566-96W 96Wells
EUR 525

OKBB00196-96W - IL-12 (p70) ELISA Kit (Human)

OKBB00196-96W 96Tests
EUR 525

Human IL-12p40(Interleukin 12 p40) ELISA Kit

ELK2263-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human IL-12p40(Interleukin 12 p40) ELISA Kit

ELK2263-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.

ELISA kit for Human IL-12 p35 (Interleukin 12 p35)

E-EL-H1647 1 plate of 96 wells
EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Human IL-12 p35 (Interleukin 12 p35) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human IL-12 p40 (Interleukin 12 p40)

E-EL-H0151 1 plate of 96 wells
EUR 452.4
Description: A sandwich ELISA kit for quantitative measurement of Human IL-12 p40 (Interleukin 12 p40) in samples from Serum, Plasma, Cell supernatant

Uncoated Human IL-12/P70-Interleukin 12/P70- ELISA Kit

E-UNEL-H0085-24Tests 24 Tests Ask for price
Description: Sandwich

Uncoated Human IL-12/P70-Interleukin 12/P70- ELISA Kit

E-UNEL-H0085-48Tests 48 Tests
EUR 958
Description: Sandwich

Uncoated Human IL-12/P70-Interleukin 12/P70- ELISA Kit

E-UNEL-H0085-96Tests 96 Tests
EUR 399
Description: Sandwich

Uncoated Human IL-12/P70-Interleukin 12/P70- ELISA Kit

E-UNEL-H0085-96Tests10 96 Tests *10
EUR 3990
Description: Sandwich

Uncoated Human IL-12/P70-Interleukin 12/P70- ELISA Kit

E-UNEL-H0085-96Tests5 96 Tests *5
EUR 1995
Description: Sandwich

Mouse IL-12/23p40 ELISA kit

E22-MC109.48 48T
EUR 295

Mouse IL-12/23p40 ELISA kit

E22-MC109.96 96T
EUR 395

IL-12/P40/ Rat IL- 12/ P40 ELISA Kit

ELA-E0058r 96 Tests
EUR 1063.2

IL-12/P70/ Rat IL- 12/ P70 ELISA Kit

ELA-E0059r 96 Tests
EUR 1063.2

Human IL-12 p70 ELISA Kit, 96 tests, Quantitative

HIL-012-125 1 kit
EUR 578.4

human IL-12p70 5 x 96

M851550005 1 unit
EUR 411.21

human IL-12p70 10 x 96

M851550010 1 unit
EUR 564.49

human IL-12p70 15 x 96

M851550015 1 unit
EUR 658.52

In addition, our data shows that ligands that trigger the secretion of IL-12P70 on TLR3 stimulation are sensitive to proteinase and some are also RNASE care. The fact that bacterial compounds such as OK432 can activate the TLR3 path in human DC are new findings. OK432 shows an important ability to induce IL-12P70 production, which is very relevant in DC-based cancer immunotherapy.

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