Quercetin induces immunoregulatory phenotype in maturing human dendritic cells.
polycyclic aromatic hydrocarbons (PAH), a group of persistent organic pollutants, associated with impaired immune function and low-grade inflammation in adults and children. However, the potential for causing a cytokine storm PAH associated with AHR (aryl hydrocarbon receptor) and NLRP3 (NLR family pyrin domain containing 3) in Human has been poorly studied. We aim to determine the relationship between exposure to PAH, AHR and NLRP3 expression, and cytokines associated with cytokine storm in healthy infants. demographic survey and a basic physical examination conducted on 248 preschoolers from electronic waste (e-waste) recycling area (Guiyu, n = 121) and the reference area (Haojiang, n = 127).
concentration of ten urinary PAH metabolites (OH-PAH) was measured. We also measure the level of expression of AHR and NLRP3 and seventeen levels of serum cytokines. several OH-PAH concentrations were significantly higher in the exposed group than in the reference group, especially 1-hydroxynaphthalene (1-OH-Nap) and 2-hydroxynaphthalene (2-OH-Nap). PAH exposure is closely related to the environment and hygiene habits of children. the level of expression of AHR and NLRP3 was significantly higher in the exposed group compared to the reference group. Similarly, serum IL -1β, IL -4, IL -5, IL -10, IL – 12p70 , IL -13, IL -17A, IL -18, < em> IL -22, IL -23, and IFN-γ were notably higher in children exposed e-waste than children of reference.
After adjusting for age, sex, BMI, family income, parental education, and traces of smoke exposure, we found that increased exposure to high PAH associated with AHR and NLRP3 expression and elevated IL -4, < em> IL -10, IL – 12p70 , IL -18, IL -22, IL -23, TNF-α, and IFN-γ levels. The relationship between PAH exposure and IL -1β, IL -18, IFN-γ and TNF-β are mediated by the expression of NLRP3, and the relationship between PAH exposure and IL -4, IL -10, IL – 12p70 , IL -22, IL -23, and TNF-α-mediated expression of AHR. Our findings indicate that the association between PAH exposure and can be mediated by cytokine storms and NLRP3 AHR expression among preschoolers.
Quercetin induces immunoregulatory phenotype in maturing human dendritic cells.
Quercetin induces immunoregulatory phenotype in maturing human dendritic cells.
Aryl hydrocarbon receptor (AHR) is the environmental sensors and ligand-activated transcription factor that is critically involved in the regulation of inflammatory responses and induction of tolerance to modulate immune cells. Such as dendritic cells (DC) express high levels of AHR, they efficiently induce immunomodulatory effects after exposure to AHR-activating compound derived from the environment or diet.
To gain new insights into the molecular target of AHR-activation following in Human monocyte-derived (mo) DC, we investigated whether natural or synthetic quercetin ligand AHR ligand 2,3,7,8-tetrachlorodibenzo p -dioxin (TCDD) to modulate the function of human moDCs regarding their ability to naive T cells prime or to migrate. Because only quercetin, but not TCDD, impaired T cell activation and migration of LPS-matured DC (LPS-DC), we analyzed the mode of action of quercetin on moDCs in more detail.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Equine Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Equine Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
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Description: A sandwich quantitative ELISA assay kit for detection of Goat Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
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Description: A sandwich quantitative ELISA assay kit for detection of Guinea pig Interleukin 1 Alpha (IL1a) in samples from serum, plasma or other biological fluids.
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Here, we find the specific down-regulation of CD83 molecule immunomodulators through direct binding of AHR is activated for CD83 promoter. In addition, treatment of LPS-DC with quercetin resulted in reduced production of pro-inflammatory cytokines IL – 12p70 and the increased expression of molecules immunoregulatory protein adapter is disabled (Dab) 2, immunoglobulin-like transcript ( IL T) -3, IL T4, IL T5 and ectonucleotidases CD39 and CD73, thereby inducing tolerogenic phenotype in the DC maturing quercetin-treated ,
