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Liver Stiffness Hinders Normalization of Systemic Inflammation and Endothelial Activation after Hepatitis C Virus (HCV) Eradication in HIV/HCV Coinfected Patients

Liver Stiffness Hinders Normalization of Systemic Inflammation and Endothelial Activation after Hepatitis C Virus (HCV) Eradication in HIV/HCV Coinfected Patients
Posted by Curtis

systemic inflammation, endothelial dysfunction and coagulopathy has high clinical relevance in the management of the living with HIV (PLHIV), and even more in patients coinfected with hepatitis C virus (HCV). It has been suggested a significant impact of HCV on these conditions. However, HCV can be eradicated in most patients with new direct-acting antiviral (DaaS) therapy. We have analyzed the effect of HCV on systemic inflammation, endothelial activation and coagulopathy in people living with HIV and its evolution after the eradication of HCV with Daas. Twenty-five HIV / HCV coinfection (/ HIV HCV group), 25 HIV monoinfected (HIV group) and 20 healthy controls (HC) were included in this study.

All patients were on antiretroviral therapy and HIV suppressed. Level fourteen signs of systemic inflammation, endothelial activation and coagulopathy ( IL -1ß, IL -6, IL – 12p70 , IL -8, TNF, D-dimer, eotaxin, IL -18, IP-10, monocyte chemotactic protein-1 (MCP-1), plasminogen activator inhibitor -1 (PAI-1), TNF receptor 1 (TNFR1), vascular cell adhesion molecule 1 (VCAM-1) and an adhesion molecule 1 (ICAM-1)) measured in the plasma at the beginning and after the eradication of HCV-mediated Daas. non-parametric tests used to establish the difference between / intra-group. At the beginning, / HIV HCV group showed increased levels of IL -18 ( p = 0.028), IP-10 ( p <0.0001) , VCAM-1 ( p <0.0001) and ICAM-1 ( p = 0.045), compared to HC and HIV groups, with the highest levels to IL 18 and IP10 observed in patients with HIV / HCV with increased liver stiffness (≥7.1 kPa).

HCV eradication Daas based therapy restored some but not all parameters evaluated. VCAM-1 remained significantly increased compared to HC ( p = 0.001), regardless of the level of the basal liver stiffness in a group of HIV / HCV, and IP-10 still increased significantly only in HIV / HCV group, with an increase in the basal level of liver stiffness compared to HC and HIV groups ( p = 0.006 and p = 0.049, respectively). These data indicate that Daas therapy in patients with HIV / HCV coinfection and HCV eradication does not always lead to the normalization of systemic inflammation and endothelial dysfunction conditions, especially in cases with an increase in liver stiffness.

Liver Stiffness Hinders Normalization of Systemic Inflammation and Endothelial Activation after Hepatitis C Virus (HCV) Eradication in HIV/HCV Coinfected Patients
Liver Stiffness Hinders Normalization of Systemic Inflammation and Endothelial Activation after Hepatitis C Virus (HCV) Eradication in HIV/HCV Coinfected Patients

Notch-mediated generation of monocyte-derived Langerhans cells: phenotype and function

Langerhans cells (LC) in the skin is the first line of defense against pathogens but also plays an important role in skin homeostasis. their exclusive expression of C-type lectin receptor Langerin / CD207 makes them prime candidates for immunotherapy. To test the vaccine, an easily accessible cell platform would be desirable as an alternative to time-consuming purification LC of Human the skin.

Here we have a model like this and show that monocytes in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF), transforming growth factor (TGF) -β1 and Notch ligand Delta-like 4 (DLL4) within 3 days differentiate into CD1a + Langerin + cells containing Birbeck granules. RNA-sequencing (RNA-seq) monocyte-derived LC’s (moLCs) confirmed the expression of genes related molecules LC, pattern recognition receptors and enhanced expression of antigen-presenting machinery.

Interleukin 10 (IL10) Polyclonal Antibody

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Description: A Rabbit polyclonal antibody against Rat Interleukin 10 (IL10)

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Description: A Rabbit polyclonal antibody against Horse Interleukin 10 (IL10)

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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10)

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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10)

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4-PAA056Ga01
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Description: A Rabbit polyclonal antibody against Gallus Interleukin 10 (IL10)

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Description: A Guinea polyclonal antibody against Rabbit Interleukin 10 (IL10)

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Description: A Rabbit polyclonal antibody against Dog Interleukin 10 (IL10). This antibody is labeled with PE.

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Description: A Rabbit polyclonal antibody against Dog Interleukin 10 (IL10). This antibody is labeled with PE.

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Description: A Rabbit polyclonal antibody against Rat Interleukin 10 (IL10). This antibody is labeled with PE.

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Description: A Rabbit polyclonal antibody against Rat Interleukin 10 (IL10). This antibody is labeled with PE.

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Description: A Rabbit polyclonal antibody against Dog Interleukin 10 (IL10). This antibody is labeled with APC.

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Description: A Rabbit polyclonal antibody against Dog Interleukin 10 (IL10). This antibody is labeled with Cy3.

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Description: A Rabbit polyclonal antibody against Dog Interleukin 10 (IL10). This antibody is labeled with HRP.

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4-PAA056Ca06-APC
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Description: A Rabbit polyclonal antibody against Dog Interleukin 10 (IL10). This antibody is labeled with APC.

Interleukin 10 (IL10) Polyclonal Antibody (Dog), Cy3

4-PAA056Ca06-Cy3
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Description: A Rabbit polyclonal antibody against Dog Interleukin 10 (IL10). This antibody is labeled with Cy3.

Interleukin 10 (IL10) Polyclonal Antibody (Dog), HRP

4-PAA056Ca06-HRP
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Description: A Rabbit polyclonal antibody against Dog Interleukin 10 (IL10). This antibody is labeled with HRP.

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Description: A Rabbit polyclonal antibody against Rat Interleukin 10 (IL10). This antibody is labeled with APC.

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Description: A Rabbit polyclonal antibody against Rat Interleukin 10 (IL10). This antibody is labeled with Cy3.

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Description: A Rabbit polyclonal antibody against Rat Interleukin 10 (IL10). This antibody is labeled with HRP.

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Description: A Rabbit polyclonal antibody against Rat Interleukin 10 (IL10). This antibody is labeled with APC.

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Description: A Rabbit polyclonal antibody against Rat Interleukin 10 (IL10). This antibody is labeled with Cy3.

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Description: A Rabbit polyclonal antibody against Rat Interleukin 10 (IL10). This antibody is labeled with HRP.

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Description: A Rabbit polyclonal antibody against Dog Interleukin 10 (IL10). This antibody is labeled with FITC.

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Description: A Rabbit polyclonal antibody against Dog Interleukin 10 (IL10). This antibody is labeled with FITC.

Interleukin 10 (IL10) Polyclonal Antibody (Horse), PE

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Description: A Rabbit polyclonal antibody against Horse Interleukin 10 (IL10). This antibody is labeled with PE.

Interleukin 10 (IL10) Polyclonal Antibody (Human), PE

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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with PE.

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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with PE.

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4-PAA056Hu06-PE
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with PE.

Interleukin 10 (IL10) Polyclonal Antibody (Mouse), PE

4-PAA056Mu01-PE
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Description: A Rabbit polyclonal antibody against Mouse Interleukin 10 (IL10). This antibody is labeled with PE.

Interleukin 10 (IL10) Polyclonal Antibody (Rat), FITC

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Description: A Rabbit polyclonal antibody against Rat Interleukin 10 (IL10). This antibody is labeled with FITC.

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4-PAA056Ra06-FITC
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Description: A Rabbit polyclonal antibody against Rat Interleukin 10 (IL10). This antibody is labeled with FITC.

Interleukin 10 (IL10) Polyclonal Antibody (Bovine), PE

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Description: A Rabbit polyclonal antibody against Bovine Interleukin 10 (IL10). This antibody is labeled with PE.

Interleukin 10 (IL10) Polyclonal Antibody (Horse), APC

4-PAA056Eq01-APC
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Description: A Rabbit polyclonal antibody against Horse Interleukin 10 (IL10). This antibody is labeled with APC.

Interleukin 10 (IL10) Polyclonal Antibody (Horse), Cy3

4-PAA056Eq01-Cy3
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Description: A Rabbit polyclonal antibody against Horse Interleukin 10 (IL10). This antibody is labeled with Cy3.

Interleukin 10 (IL10) Polyclonal Antibody (Horse), HRP

4-PAA056Eq01-HRP
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Description: A Rabbit polyclonal antibody against Horse Interleukin 10 (IL10). This antibody is labeled with HRP.

Interleukin 10 (IL10) Polyclonal Antibody (Gallus), PE

4-PAA056Ga01-PE
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Description: A Rabbit polyclonal antibody against Gallus Interleukin 10 (IL10). This antibody is labeled with PE.

Interleukin 10 (IL10) Polyclonal Antibody (Human), APC

4-PAA056Hu01-APC
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with APC.

Interleukin 10 (IL10) Polyclonal Antibody (Human), Cy3

4-PAA056Hu01-Cy3
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with Cy3.

Interleukin 10 (IL10) Polyclonal Antibody (Human), HRP

4-PAA056Hu01-HRP
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with HRP.

Interleukin 10 (IL10) Polyclonal Antibody (Human), APC

4-PAA056Hu02-APC
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with APC.

Interleukin 10 (IL10) Polyclonal Antibody (Human), Cy3

4-PAA056Hu02-Cy3
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with Cy3.

Interleukin 10 (IL10) Polyclonal Antibody (Human), HRP

4-PAA056Hu02-HRP
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with HRP.

Interleukin 10 (IL10) Polyclonal Antibody (Human), APC

4-PAA056Hu06-APC
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with APC.

Interleukin 10 (IL10) Polyclonal Antibody (Human), Cy3

4-PAA056Hu06-Cy3
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with Cy3.

Interleukin 10 (IL10) Polyclonal Antibody (Human), HRP

4-PAA056Hu06-HRP
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with HRP.

Interleukin 10 (IL10) Polyclonal Antibody (Mouse), APC

4-PAA056Mu01-APC
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Description: A Rabbit polyclonal antibody against Mouse Interleukin 10 (IL10). This antibody is labeled with APC.

Interleukin 10 (IL10) Polyclonal Antibody (Mouse), Cy3

4-PAA056Mu01-Cy3
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Description: A Rabbit polyclonal antibody against Mouse Interleukin 10 (IL10). This antibody is labeled with Cy3.

Interleukin 10 (IL10) Polyclonal Antibody (Mouse), HRP

4-PAA056Mu01-HRP
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Description: A Rabbit polyclonal antibody against Mouse Interleukin 10 (IL10). This antibody is labeled with HRP.

Interleukin 10 (IL10) Polyclonal Antibody (Rabbit), PE

4-PAA056Rb51-PE
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Description: A Guinea polyclonal antibody against Rabbit Interleukin 10 (IL10). This antibody is labeled with PE.

Interleukin 10 (IL10) Polyclonal Antibody (Bovine), APC

4-PAA056Bo01-APC
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Description: A Rabbit polyclonal antibody against Bovine Interleukin 10 (IL10). This antibody is labeled with APC.

Interleukin 10 (IL10) Polyclonal Antibody (Bovine), Cy3

4-PAA056Bo01-Cy3
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Description: A Rabbit polyclonal antibody against Bovine Interleukin 10 (IL10). This antibody is labeled with Cy3.

Interleukin 10 (IL10) Polyclonal Antibody (Bovine), HRP

4-PAA056Bo01-HRP
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Description: A Rabbit polyclonal antibody against Bovine Interleukin 10 (IL10). This antibody is labeled with HRP.

Interleukin 10 (IL10) Polyclonal Antibody (Horse), FITC

4-PAA056Eq01-FITC
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Description: A Rabbit polyclonal antibody against Horse Interleukin 10 (IL10). This antibody is labeled with FITC.

Interleukin 10 (IL10) Polyclonal Antibody (Gallus), APC

4-PAA056Ga01-APC
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Description: A Rabbit polyclonal antibody against Gallus Interleukin 10 (IL10). This antibody is labeled with APC.

Interleukin 10 (IL10) Polyclonal Antibody (Gallus), Cy3

4-PAA056Ga01-Cy3
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  • 100ul
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Description: A Rabbit polyclonal antibody against Gallus Interleukin 10 (IL10). This antibody is labeled with Cy3.

Interleukin 10 (IL10) Polyclonal Antibody (Gallus), HRP

4-PAA056Ga01-HRP
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  • 100ul
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Description: A Rabbit polyclonal antibody against Gallus Interleukin 10 (IL10). This antibody is labeled with HRP.

Interleukin 10 (IL10) Polyclonal Antibody (Human), FITC

4-PAA056Hu01-FITC
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with FITC.

Interleukin 10 (IL10) Polyclonal Antibody (Human), FITC

4-PAA056Hu02-FITC
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with FITC.

Interleukin 10 (IL10) Polyclonal Antibody (Human), FITC

4-PAA056Hu06-FITC
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  • 100ul
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with FITC.

Interleukin 10 (IL10) Polyclonal Antibody (Mouse), FITC

4-PAA056Mu01-FITC
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  • 100ul
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Description: A Rabbit polyclonal antibody against Mouse Interleukin 10 (IL10). This antibody is labeled with FITC.

Interleukin 10 (IL10) Polyclonal Antibody (Rabbit), APC

4-PAA056Rb51-APC
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  • 100ul
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Description: A Guinea polyclonal antibody against Rabbit Interleukin 10 (IL10). This antibody is labeled with APC.

Interleukin 10 (IL10) Polyclonal Antibody (Rabbit), Cy3

4-PAA056Rb51-Cy3
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  • 100ul
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Description: A Guinea polyclonal antibody against Rabbit Interleukin 10 (IL10). This antibody is labeled with Cy3.

Interleukin 10 (IL10) Polyclonal Antibody (Rabbit), HRP

4-PAA056Rb51-HRP
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  • 100ul
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Description: A Guinea polyclonal antibody against Rabbit Interleukin 10 (IL10). This antibody is labeled with HRP.

Interleukin 10 (IL10) Polyclonal Antibody (Bovine), FITC

4-PAA056Bo01-FITC
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  • 100ul
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Description: A Rabbit polyclonal antibody against Bovine Interleukin 10 (IL10). This antibody is labeled with FITC.

Interleukin 10 (IL10) Polyclonal Antibody (Gallus), FITC

4-PAA056Ga01-FITC
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  • 100ul
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Description: A Rabbit polyclonal antibody against Gallus Interleukin 10 (IL10). This antibody is labeled with FITC.

Interleukin 10 (IL10) Polyclonal Antibody (Rabbit), FITC

4-PAA056Rb51-FITC
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Description: A Guinea polyclonal antibody against Rabbit Interleukin 10 (IL10). This antibody is labeled with FITC.

Interleukin 10 (IL10) Polyclonal Antibody (Dog), Biotinylated

4-PAA056Ca01-Biotin
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Description: A Rabbit polyclonal antibody against Dog Interleukin 10 (IL10). This antibody is labeled with Biotin.

Interleukin 10 (IL10) Polyclonal Antibody (Dog), Biotinylated

4-PAA056Ca06-Biotin
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Description: A Rabbit polyclonal antibody against Dog Interleukin 10 (IL10). This antibody is labeled with Biotin.

Interleukin 10 (IL10) Polyclonal Antibody (Rat), Biotinylated

4-PAA056Ra01-Biotin
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Description: A Rabbit polyclonal antibody against Rat Interleukin 10 (IL10). This antibody is labeled with Biotin.

Interleukin 10 (IL10) Polyclonal Antibody (Rat), Biotinylated

4-PAA056Ra06-Biotin
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Description: A Rabbit polyclonal antibody against Rat Interleukin 10 (IL10). This antibody is labeled with Biotin.

Interleukin 10 (IL10) Polyclonal Antibody (Dog), APC-Cy7

4-PAA056Ca01-APC-Cy7
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Description: A Rabbit polyclonal antibody against Dog Interleukin 10 (IL10). This antibody is labeled with APC-Cy7.

Interleukin 10 (IL10) Polyclonal Antibody (Dog), APC-Cy7

4-PAA056Ca06-APC-Cy7
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Description: A Rabbit polyclonal antibody against Dog Interleukin 10 (IL10). This antibody is labeled with APC-Cy7.

Interleukin 10 (IL10) Polyclonal Antibody (Rat), APC-Cy7

4-PAA056Ra01-APC-Cy7
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Description: A Rabbit polyclonal antibody against Rat Interleukin 10 (IL10). This antibody is labeled with APC-Cy7.

Interleukin 10 (IL10) Polyclonal Antibody (Rat), APC-Cy7

4-PAA056Ra06-APC-Cy7
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Description: A Rabbit polyclonal antibody against Rat Interleukin 10 (IL10). This antibody is labeled with APC-Cy7.

Interleukin 10 (IL10) Polyclonal Antibody (Horse), Biotinylated

4-PAA056Eq01-Biotin
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Description: A Rabbit polyclonal antibody against Horse Interleukin 10 (IL10). This antibody is labeled with Biotin.

Interleukin 10 (IL10) Polyclonal Antibody (Human), Biotinylated

4-PAA056Hu01-Biotin
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with Biotin.

Interleukin 10 (IL10) Polyclonal Antibody (Human), Biotinylated

4-PAA056Hu02-Biotin
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with Biotin.

Interleukin 10 (IL10) Polyclonal Antibody (Human), Biotinylated

4-PAA056Hu06-Biotin
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with Biotin.

Interleukin 10 (IL10) Polyclonal Antibody (Mouse), Biotinylated

4-PAA056Mu01-Biotin
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Description: A Rabbit polyclonal antibody against Mouse Interleukin 10 (IL10). This antibody is labeled with Biotin.

Interleukin 10 (IL10) Polyclonal Antibody (Horse), APC-Cy7

4-PAA056Eq01-APC-Cy7
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Description: A Rabbit polyclonal antibody against Horse Interleukin 10 (IL10). This antibody is labeled with APC-Cy7.

Interleukin 10 (IL10) Polyclonal Antibody (Human), APC-Cy7

4-PAA056Hu01-APC-Cy7
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with APC-Cy7.

Interleukin 10 (IL10) Polyclonal Antibody (Human), APC-Cy7

4-PAA056Hu02-APC-Cy7
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with APC-Cy7.

Interleukin 10 (IL10) Polyclonal Antibody (Human), APC-Cy7

4-PAA056Hu06-APC-Cy7
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Description: A Rabbit polyclonal antibody against Human Interleukin 10 (IL10). This antibody is labeled with APC-Cy7.

Interleukin 10 (IL10) Polyclonal Antibody (Mouse), APC-Cy7

4-PAA056Mu01-APC-Cy7
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Description: A Rabbit polyclonal antibody against Mouse Interleukin 10 (IL10). This antibody is labeled with APC-Cy7.

Interleukin 10 (IL10) Polyclonal Antibody (Bovine), Biotinylated

4-PAA056Bo01-Biotin
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Description: A Rabbit polyclonal antibody against Bovine Interleukin 10 (IL10). This antibody is labeled with Biotin.

Interleukin 10 (IL10) Polyclonal Antibody (Gallus), Biotinylated

4-PAA056Ga01-Biotin
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  • 100ul
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Description: A Rabbit polyclonal antibody against Gallus Interleukin 10 (IL10). This antibody is labeled with Biotin.

Interleukin 10 (IL10) Polyclonal Antibody (Rabbit), Biotinylated

4-PAA056Rb51-Biotin
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Description: A Guinea polyclonal antibody against Rabbit Interleukin 10 (IL10). This antibody is labeled with Biotin.

Interleukin 10 (IL10) Polyclonal Antibody (Bovine), APC-Cy7

4-PAA056Bo01-APC-Cy7
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  • 100ul
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Description: A Rabbit polyclonal antibody against Bovine Interleukin 10 (IL10). This antibody is labeled with APC-Cy7.

Interleukin 10 (IL10) Polyclonal Antibody (Gallus), APC-Cy7

4-PAA056Ga01-APC-Cy7
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  • 100ul
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Description: A Rabbit polyclonal antibody against Gallus Interleukin 10 (IL10). This antibody is labeled with APC-Cy7.

Interleukin 10 (IL10) Polyclonal Antibody (Rabbit), APC-Cy7

4-PAA056Rb51-APC-Cy7
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  • 100ul
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Description: A Guinea polyclonal antibody against Rabbit Interleukin 10 (IL10). This antibody is labeled with APC-Cy7.

Interleukin 18 (IL18) Polyclonal Antibody

CAU28766-100ul 100ul
EUR 204.5

Interleukin 18 (IL18) Polyclonal Antibody

CAU28766-200ul 200ul
EUR 255.4

Interleukin 18 (IL18) Polyclonal Antibody

CAU28767-100ul 100ul
EUR 254.4

Interleukin 18 (IL18) Polyclonal Antibody

CAU28767-200ul 200ul
EUR 317.5

Interleukin 18 (IL18) Polyclonal Antibody

CAU28768-100ul 100ul
EUR 217.9

Interleukin 18 (IL18) Polyclonal Antibody

CAU28768-200ul 200ul
EUR 272.2

Interleukin 18 (IL18) Polyclonal Antibody

CAU28769-100ul 100ul
EUR 188.2

Interleukin 18 (IL18) Polyclonal Antibody

CAU28769-200ul 200ul
EUR 235.2

Interleukin 18 (IL18) Polyclonal Antibody

CAU28770-100ul 100ul
EUR 229.4

Interleukin 18 (IL18) Polyclonal Antibody

CAU28770-200ul 200ul
EUR 287.3

Interleukin 18 (IL18) Polyclonal Antibody

CAU28771-100ul 100ul
EUR 241.9

Interleukin 18 (IL18) Polyclonal Antibody

CAU28771-200ul 200ul
EUR 302.4

Interleukin 18 (IL18) Polyclonal Antibody

CAU28772-100ul 100ul
EUR 254.4

At the protein level, moLCs showed low expression costimulatory molecule but an expression that stands out from the type C lectin receptors. MoLCs can mature, secrete IL – 12p70 and TNF-α and stimulates proliferation and cytokine production in allogeneic CD4 + and CD8 + < / sup> T cell vaccine in terms of testing, a Langerin glycomimetic recently marked ligand conjugate liposomes showed specific and rapid internalization into moLCs. Therefore, these short-term moLCs vitro produced an interesting tool to screen LC-based vaccines in the future.

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