Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is predominantly type 2 inflammatory disease of the upper air ways. A subgroup of patients with CRSwNP suffering from intolerance to nonsteroidal anti-inflammatory drugs (NSAIDs) and developing NSAID-exacerbated respiratory disease (NERD). The purpose of this study was to compare by nasal secretion of inflammatory cytokines endotype CRSwNP patients with and without NSAIDs intolerance.
Method: 20-6 collected nasal secretions of patients suffering from CRSwNP, thirteen with NERD and thirteen without NSAIDs intolerance. As a control, nasal secretions were collected from a healthy donor fifteen. Tryptase and ten Human cyto- kines analyzed: interleukin ( IL ) – 4, IL -5, IL – 6, IL -8, IL – 12p70 , IL -13, IL -17A, IL -23, IFN-g and TNF-a by cytokines multiple arrays on the Luminex 200 platform.
Results: Grade of polyposis and more severe operating frequency of polyps in NERD- compared with non-NERD patients. IL-6 and IL-5 in CRSwNP significantly increased compared to the healthy volunteers. IL-5 and IL-13 were significantly increased in subjects suffering from NERD compared with patients without NERD CRSwNP. Conclusion: We identified IL-13 as a biomarker may be limited in the nasal secretions of patients with NERD, which allows us to distinguish between CRSwNP with vs without NERD. Characterization endotypes inflammation in CRSwNP allow the introduction of the best available therapy in the context of precision medicine.
Endotypes of chronic rhinosinusitis with nasal polyps with and without NSAID – intolerance
Mesenchymal Stem Cell Derived biocompatible membrane vesicles Demonstrate Immunomodulatory Activity Inhibiting activation and proliferation of human mononuclear cells
immune-mediated disease characterized by the abnormal activity of the immune system. B-induced membrane vesicles Cytochalasin (CIMVs) is an innovative therapy instrument. However, the immunomodulatory activity of Human mesenchymal stem cell (MSC) -derived CIMVs (CIMVs-MSCs) are still unknown.
Therefore, we sought to investigate the immunological properties of CIMVs-MSCs and evaluate its effect on Human peripheral blood mononuclear cells (PBMC). We found that CIMVs-MSCs mainly uptaken by monocytes and B-cells. In addition, we show that CIMVs-MSCs inhibit phytohemagglutinin (PHA) induced proliferation of PBMC, with a more pronounced effect on the expansion of T-lymphocytes compared to the B-cell. In addition, the activation of T-helper (CD4 + CD25 +), B-cells (CD19 + CD25 +) and T-cytotoxic lymphocytes (CD8 + CD25 +) were also significantly suppressed by CIMVs-MSC.
Interleukin 4 (IL4) Polyclonal Antibody
MBS2032130-01mL
MyBiosource
0.1mL
EUR 140
Interleukin 4 (IL4) Polyclonal Antibody
MBS2032130-02mL
MyBiosource
0.2mL
EUR 165
Interleukin 4 (IL4) Polyclonal Antibody
MBS2032130-05mL
MyBiosource
0.5mL
EUR 240
Interleukin 4 (IL4) Polyclonal Antibody
MBS2032130-1mL
MyBiosource
1mL
EUR 285
Interleukin 4 (IL4) Polyclonal Antibody
MBS2032130-5mL
MyBiosource
5mL
EUR 735
Interleukin 4 (IL4) Polyclonal Antibody
MBS2032196-01mL
MyBiosource
0.1mL
EUR 145
Interleukin 4 (IL4) Polyclonal Antibody
MBS2032196-02mL
MyBiosource
0.2mL
EUR 170
Interleukin 4 (IL4) Polyclonal Antibody
MBS2032196-05mL
MyBiosource
0.5mL
EUR 260
Interleukin 4 (IL4) Polyclonal Antibody
MBS2032196-1mL
MyBiosource
1mL
EUR 300
Interleukin 4 (IL4) Polyclonal Antibody
MBS2032196-5mL
MyBiosource
5mL
EUR 800
Interleukin 4 (IL4) Polyclonal Antibody
MBS2032535-01mL
MyBiosource
0.1mL
EUR 140
Interleukin 4 (IL4) Polyclonal Antibody
MBS2032535-02mL
MyBiosource
0.2mL
EUR 165
Interleukin 4 (IL4) Polyclonal Antibody
MBS2032535-05mL
MyBiosource
0.5mL
EUR 240
Interleukin 4 (IL4) Polyclonal Antibody
MBS2032535-1mL
MyBiosource
1mL
EUR 280
Interleukin 4 (IL4) Polyclonal Antibody
MBS2032535-5mL
MyBiosource
5mL
EUR 730
Interleukin 4 (IL4) Polyclonal Antibody (Dog)
4-PAA077Ca01
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4-PAA077Po01
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Interleukin 4 (IL4) Polyclonal Antibody (Rat)
4-PAA077Ra01
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Description: A Rabbit polyclonal antibody against Rat Interleukin 4 (IL4)
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4-PAA077Ra06
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4-PAA077Hu01
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4-PAA077Hu02
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4-PAA077Hu06
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4-PAA077Mu01
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4-PAA077Mu06
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4-PAA077Rb51
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4-PAA077Ga01
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Polyclonal Antibody to Interleukin 4 (IL4)
PAA077Ca01
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PAA077Ga01
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PAA077Gu01
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PAA077Hu01
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PAA077Hu02
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PAA077Mu01
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PAA077Mu02
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PAA077Po01
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PAA077Ra01
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Polyclonal Antibody to Interleukin 4 (IL4)
PAA077Rb51
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Polyclonal Antibody to Interleukin 4 (IL4)
MBS2014299-01mL
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MBS2014299-05mL
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MBS2014299-1mL
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MBS2014299-5mL
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Polyclonal Antibody to Interleukin 4 (IL4)
MBS2005933-01mL
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EUR 140
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MBS2005933-02mL
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EUR 165
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2005933-05mL
MyBiosource
0.5mL
EUR 240
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2005933-1mL
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1mL
EUR 280
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2005933-5mL
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5mL
EUR 730
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2028668-01mL
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EUR 170
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MBS2028668-02mL
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EUR 215
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2028668-05mL
MyBiosource
0.5mL
EUR 350
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2028668-1mL
MyBiosource
1mL
EUR 425
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2028668-5mL
MyBiosource
5mL
EUR 1160
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2027100-01mL
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EUR 170
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MBS2027100-02mL
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EUR 205
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MBS2027100-05mL
MyBiosource
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EUR 330
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MBS2027100-1mL
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1mL
EUR 405
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2027100-5mL
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5mL
EUR 1100
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2027146-01mL
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EUR 175
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MBS2027146-02mL
MyBiosource
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EUR 220
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MBS2027146-05mL
MyBiosource
0.5mL
EUR 365
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2027146-1mL
MyBiosource
1mL
EUR 445
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2027146-5mL
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5mL
EUR 1220
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2033891-01mL
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EUR 175
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2033891-02mL
MyBiosource
0.2mL
EUR 220
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2033891-05mL
MyBiosource
0.5mL
EUR 360
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2033891-1mL
MyBiosource
1mL
EUR 440
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2033891-5mL
MyBiosource
5mL
EUR 1205
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2111765-01mL
MyBiosource
0.1mL
EUR 175
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2111765-02mL
MyBiosource
0.2mL
EUR 220
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2111765-05mL
MyBiosource
0.5mL
EUR 365
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2111765-1mL
MyBiosource
1mL
EUR 445
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2111765-5mL
MyBiosource
5mL
EUR 1220
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2007157-01mL
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0.1mL
EUR 170
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2007157-02mL
MyBiosource
0.2mL
EUR 215
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2007157-05mL
MyBiosource
0.5mL
EUR 345
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2007157-1mL
MyBiosource
1mL
EUR 420
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2007157-5mL
MyBiosource
5mL
EUR 1155
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2005290-01mL
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0.1mL
EUR 170
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2005290-02mL
MyBiosource
0.2mL
EUR 215
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2005290-05mL
MyBiosource
0.5mL
EUR 350
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2005290-1mL
MyBiosource
1mL
EUR 425
Polyclonal Antibody to Interleukin 4 (IL4)
MBS2005290-5mL
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5mL
EUR 1160
Interleukin 4 (IL4) Polyclonal Antibody (Dog), PE
4-PAA077Ca01-PE
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Description: A Rabbit polyclonal antibody against Dog Interleukin 4 (IL4). This antibody is labeled with PE.
Interleukin 4 (IL4) Polyclonal Antibody (Pig), PE
4-PAA077Po01-PE
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Description: A Rabbit polyclonal antibody against Pig Interleukin 4 (IL4). This antibody is labeled with PE.
Interleukin 4 (IL4) Polyclonal Antibody (Rat), PE
4-PAA077Ra01-PE
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Description: A Rabbit polyclonal antibody against Rat Interleukin 4 (IL4). This antibody is labeled with PE.
Interleukin 4 (IL4) Polyclonal Antibody (Rat), PE
4-PAA077Ra06-PE
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Description: A Rabbit polyclonal antibody against Rat Interleukin 4 (IL4). This antibody is labeled with PE.
Interleukin 4 (IL4) Polyclonal Antibody (Dog), APC
4-PAA077Ca01-APC
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Description: A Rabbit polyclonal antibody against Dog Interleukin 4 (IL4). This antibody is labeled with APC.
Interleukin 4 (IL4) Polyclonal Antibody (Dog), Cy3
4-PAA077Ca01-Cy3
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Description: A Rabbit polyclonal antibody against Dog Interleukin 4 (IL4). This antibody is labeled with Cy3.
Interleukin 4 (IL4) Polyclonal Antibody (Dog), HRP
4-PAA077Ca01-HRP
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Description: A Rabbit polyclonal antibody against Dog Interleukin 4 (IL4). This antibody is labeled with HRP.
Interleukin 4 (IL4) Polyclonal Antibody (Pig), APC
4-PAA077Po01-APC
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Description: A Rabbit polyclonal antibody against Pig Interleukin 4 (IL4). This antibody is labeled with APC.
Interleukin 4 (IL4) Polyclonal Antibody (Pig), Cy3
4-PAA077Po01-Cy3
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Description: A Rabbit polyclonal antibody against Pig Interleukin 4 (IL4). This antibody is labeled with Cy3.
Interleukin 4 (IL4) Polyclonal Antibody (Pig), HRP
4-PAA077Po01-HRP
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Description: A Rabbit polyclonal antibody against Pig Interleukin 4 (IL4). This antibody is labeled with HRP.
Interleukin 4 (IL4) Polyclonal Antibody (Rat), APC
4-PAA077Ra01-APC
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Description: A Rabbit polyclonal antibody against Rat Interleukin 4 (IL4). This antibody is labeled with APC.
Interleukin 4 (IL4) Polyclonal Antibody (Rat), Cy3
4-PAA077Ra01-Cy3
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Description: A Rabbit polyclonal antibody against Rat Interleukin 4 (IL4). This antibody is labeled with Cy3.
Interleukin 4 (IL4) Polyclonal Antibody (Rat), HRP
4-PAA077Ra01-HRP
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Description: A Rabbit polyclonal antibody against Rat Interleukin 4 (IL4). This antibody is labeled with HRP.
Interleukin 4 (IL4) Polyclonal Antibody (Rat), APC
4-PAA077Ra06-APC
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Description: A Rabbit polyclonal antibody against Rat Interleukin 4 (IL4). This antibody is labeled with APC.
Interleukin 4 (IL4) Polyclonal Antibody (Rat), Cy3
4-PAA077Ra06-Cy3
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Description: A Rabbit polyclonal antibody against Rat Interleukin 4 (IL4). This antibody is labeled with Cy3.
Interleukin 4 (IL4) Polyclonal Antibody (Rat), HRP
4-PAA077Ra06-HRP
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Description: A Rabbit polyclonal antibody against Rat Interleukin 4 (IL4). This antibody is labeled with HRP.
Interleukin 4 (IL4) Polyclonal Antibody (Dog), FITC
4-PAA077Ca01-FITC
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Description: A Rabbit polyclonal antibody against Dog Interleukin 4 (IL4). This antibody is labeled with FITC.
Interleukin 4 (IL4) Polyclonal Antibody (Human), PE
4-PAA077Hu01-PE
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Description: A Rabbit polyclonal antibody against Human Interleukin 4 (IL4). This antibody is labeled with PE.
Interleukin 4 (IL4) Polyclonal Antibody (Human), PE
4-PAA077Hu02-PE
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Description: A Rabbit polyclonal antibody against Human Interleukin 4 (IL4). This antibody is labeled with PE.
Interleukin 4 (IL4) Polyclonal Antibody (Human), PE
4-PAA077Hu06-PE
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Description: A Rabbit polyclonal antibody against Human Interleukin 4 (IL4). This antibody is labeled with PE.
Interleukin 4 (IL4) Polyclonal Antibody (Mouse), PE
4-PAA077Mu01-PE
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Description: A Rabbit polyclonal antibody against Mouse Interleukin 4 (IL4). This antibody is labeled with PE.
Interleukin 4 (IL4) Polyclonal Antibody (Mouse), PE
4-PAA077Mu06-PE
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100ul
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Description: A Rabbit polyclonal antibody against Mouse Interleukin 4 (IL4). This antibody is labeled with PE.
Interleukin 4 (IL4) Polyclonal Antibody (Pig), FITC
4-PAA077Po01-FITC
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Description: A Rabbit polyclonal antibody against Pig Interleukin 4 (IL4). This antibody is labeled with FITC.
Interleukin 4 (IL4) Polyclonal Antibody (Rat), FITC
4-PAA077Ra01-FITC
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100ul
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Description: A Rabbit polyclonal antibody against Rat Interleukin 4 (IL4). This antibody is labeled with FITC.
Interleukin 4 (IL4) Polyclonal Antibody (Rat), FITC
4-PAA077Ra06-FITC
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Description: A Rabbit polyclonal antibody against Rat Interleukin 4 (IL4). This antibody is labeled with FITC.
Interleukin 4 (IL4) Polyclonal Antibody (Human), APC
4-PAA077Hu01-APC
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Description: A Rabbit polyclonal antibody against Human Interleukin 4 (IL4). This antibody is labeled with APC.
Interleukin 4 (IL4) Polyclonal Antibody (Human), Cy3
4-PAA077Hu01-Cy3
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100ul
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Description: A Rabbit polyclonal antibody against Human Interleukin 4 (IL4). This antibody is labeled with Cy3.
Interleukin 4 (IL4) Polyclonal Antibody (Human), HRP
4-PAA077Hu01-HRP
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Description: A Rabbit polyclonal antibody against Human Interleukin 4 (IL4). This antibody is labeled with HRP.
Interleukin 4 (IL4) Polyclonal Antibody (Human), APC
4-PAA077Hu02-APC
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100ul
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Description: A Rabbit polyclonal antibody against Human Interleukin 4 (IL4). This antibody is labeled with APC.
Interleukin 4 (IL4) Polyclonal Antibody (Human), Cy3
4-PAA077Hu02-Cy3
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100ul
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Description: A Rabbit polyclonal antibody against Human Interleukin 4 (IL4). This antibody is labeled with Cy3.
Interleukin 4 (IL4) Polyclonal Antibody (Human), HRP
4-PAA077Hu02-HRP
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100ul
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Description: A Rabbit polyclonal antibody against Human Interleukin 4 (IL4). This antibody is labeled with HRP.
Interleukin 4 (IL4) Polyclonal Antibody (Human), APC
4-PAA077Hu06-APC
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100ul
10ml
1ml
200ul
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Description: A Rabbit polyclonal antibody against Human Interleukin 4 (IL4). This antibody is labeled with APC.
Interleukin 4 (IL4) Polyclonal Antibody (Human), Cy3
4-PAA077Hu06-Cy3
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100ul
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1ml
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Description: A Rabbit polyclonal antibody against Human Interleukin 4 (IL4). This antibody is labeled with Cy3.
Interleukin 4 (IL4) Polyclonal Antibody (Human), HRP
4-PAA077Hu06-HRP
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100ul
10ml
1ml
200ul
20ul
Description: A Rabbit polyclonal antibody against Human Interleukin 4 (IL4). This antibody is labeled with HRP.
Interleukin 4 (IL4) Polyclonal Antibody (Mouse), APC
4-PAA077Mu01-APC
Cloud-Clone
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100ul
10ml
1ml
200ul
20ul
Description: A Rabbit polyclonal antibody against Mouse Interleukin 4 (IL4). This antibody is labeled with APC.
Interleukin 4 (IL4) Polyclonal Antibody (Mouse), Cy3
4-PAA077Mu01-Cy3
Cloud-Clone
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100ul
10ml
1ml
200ul
20ul
Description: A Rabbit polyclonal antibody against Mouse Interleukin 4 (IL4). This antibody is labeled with Cy3.
Interleukin 4 (IL4) Polyclonal Antibody (Mouse), HRP
4-PAA077Mu01-HRP
Cloud-Clone
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100ul
10ml
1ml
200ul
20ul
Description: A Rabbit polyclonal antibody against Mouse Interleukin 4 (IL4). This antibody is labeled with HRP.
Interleukin 4 (IL4) Polyclonal Antibody (Mouse), APC
4-PAA077Mu06-APC
Cloud-Clone
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100ul
10ml
1ml
200ul
20ul
Description: A Rabbit polyclonal antibody against Mouse Interleukin 4 (IL4). This antibody is labeled with APC.
Interleukin 4 (IL4) Polyclonal Antibody (Mouse), Cy3
4-PAA077Mu06-Cy3
Cloud-Clone
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100ul
10ml
1ml
200ul
20ul
Description: A Rabbit polyclonal antibody against Mouse Interleukin 4 (IL4). This antibody is labeled with Cy3.
Interleukin 4 (IL4) Polyclonal Antibody (Mouse), HRP
4-PAA077Mu06-HRP
Cloud-Clone
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100ul
10ml
1ml
200ul
20ul
Description: A Rabbit polyclonal antibody against Mouse Interleukin 4 (IL4). This antibody is labeled with HRP.
Interleukin 4 (IL4) Polyclonal Antibody (Rabbit), PE
4-PAA077Rb51-PE
Cloud-Clone
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100ul
10ml
1ml
200ul
20ul
Description: A Guinea polyclonal antibody against Rabbit Interleukin 4 (IL4). This antibody is labeled with PE.
Interleukin 4 (IL4) Polyclonal Antibody (Chicken), PE
4-PAA077Ga01-PE
Cloud-Clone
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100ul
10ml
1ml
200ul
20ul
Description: A Rabbit polyclonal antibody against Chicken Interleukin 4 (IL4). This antibody is labeled with PE.
Interleukin 4 (IL4) Polyclonal Antibody (Human), FITC
4-PAA077Hu01-FITC
Cloud-Clone
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100ul
10ml
1ml
200ul
20ul
Description: A Rabbit polyclonal antibody against Human Interleukin 4 (IL4). This antibody is labeled with FITC.
Interleukin 4 (IL4) Polyclonal Antibody (Human), FITC
4-PAA077Hu02-FITC
Cloud-Clone
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100ul
10ml
1ml
200ul
20ul
Description: A Rabbit polyclonal antibody against Human Interleukin 4 (IL4). This antibody is labeled with FITC.
Additionally, CIMVs-MSCs decrease in the secretion of a factor of epidermal growth (EGF) and fractalkine pro-inflammatory in the population of PBMCs, while the release of FGF-2, G-CSF, anti-inflammatory GM-CSF, MCP-3, anti-inflammatory MDC, anti -inflamasi IL – 12p70 , a pro-inflammatory IL -1b, and MCP-1 increased. We analyzed the effect of CIMVs-MSCs in isolated populations of CD4 + and CD8 + T-lymphocytes and show a different immune response and cytokine secretion. Finally, we observe that there is no xenogeneic or allogeneic transplantation of CIMVs induced immune responses in mice. Our data show that CIMVs-MSCs have immunosuppressive properties, is a potential agent for immunomodulatory treatment, and worthy of further investigation.