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Enhanced Human T Lymphocyte Antigen Priming by Cytokine-Matured Dendritic Cells Overexpressing Bcl-2 and IL-12.

Enhanced Human T Lymphocyte Antigen Priming by Cytokine-Matured Dendritic Cells Overexpressing Bcl-2 and IL-12.
Posted by Curtis

dendritic cell (DC) based vaccine is a promising strategy for cancer immunotherapy. However, clinical trials have shown only limited efficacy, demonstrates the need to optimize protocols for Human DC vaccine preparation. In this study, we systematically compared five different Human vaccines DC maturation protocols used in clinical trials: (1) four-cytokine cocktail (TNF-α, IL -6, IL -1β, and PGE2); (2) cocktails α-DC-cytokine (TNF-α, IL -1β, IFN-α, IFN-γ, and poly I: C); (3) lipopolysaccharide (LPS) / IFN-γ; (4) TNF-α and PGE2; and (5) Trimix (mRNA encoding CD40L, CD70, and constitutively activated Toll-like receptor 4 electroporated into immature DC).

We found that four cytokine cocktail induced high levels of costimulatory and HLA molecules, and CCR7, in DC. Mature DC (MDCs) matured with a cocktail of four cytokines have higher viability than those obtained with other protocols. Based on these features, we chose four cytokine cocktail protocols to further enhance the ability of immunization of DC by introducing exogenous genes. We show that the introduction of exogenous Bcl-2 increased DC survival. Furthermore, introducing the IL – 12p70 rescued inhibition of IL -12 secretion by PGE2 without damaging the DC phenotype.

Introducing both Bcl-2 and IL – 12p70 mRNA into DC induced enhanced cytomegalovirus pp65-specific CD8 + T cells secreting IFN-γ and TNF-α , Taken together, our data demonstrate that DCs matured by a cocktail of four cytokines combined with exogenous Bcl-2 and IL – 12p70 gene expression is a promising approach for clinical applications in cancer immunotherapy.

Enhanced Human T Lymphocyte Antigen Priming by Cytokine-Matured Dendritic Cells Overexpressing Bcl-2 and IL-12.
Enhanced Human T Lymphocyte Antigen Priming by Cytokine-Matured Dendritic Cells Overexpressing Bcl-2 and IL-12.

Mycobacterium avium subsp. paratuberculosis MAP1889c Protein Induces Maturation of Dendritic cells and Th2-biased Drives Immune Responses.

Mycobacterium avium subsp . paratuberculosis (MAP) is the causative agent of chronic granulomatous bowel disease in animals and is associated with various autoimmune diseases in Human including Crohn’s disease. A good understanding of host-protective immune response and antibacterial immunity is controlled by MAP and its components may contribute to the development of effective control strategies.

MAP1889c seroreactive identified as an antigen in patients with Crohn’s disease. In this study, we investigated the immunological function of MAP1889c in dendritic cells (DC). MAP1889c stimulated DC to increase the expression of molecular co-stimulatory (CD80 and CD86) and molecular complex class major histocompatibility (MHC) and the secret interleukin higher ( IL ) – 10 and moderate IL -6, tumor necrosis factor (TNF) -α, and IL – 12p70 level through Toll-like receptor (TLR) 4 lanes.

MAP1889c-induced DC activation mediated by mitogen-activated protein kinases (MAPKs), cAMPp-response element binding protein (CREB), and nuclear factor kappa B (NF-kB). In particular, the signal is essential for MAP1889c CREB-mediated IL -10 production but not TNF-α and IL – 12p70 . In addition, DC-mature MAP1889c induced T cell proliferation and drove Th2 response.

Canine Interleukin 1 Alpha (IL1a) ELISA Kit

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Description: A sandwich quantitative ELISA assay kit for detection of Canine Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

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Description: A sandwich quantitative ELISA assay kit for detection of Equine Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Equine Interleukin 1 Alpha (IL1a) ELISA Kit

DLR-IL1a-Eq-96T 96T
EUR 654
Description: A sandwich quantitative ELISA assay kit for detection of Equine Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Goat Interleukin 1 Alpha (IL1a) ELISA Kit

DLR-IL1a-g-48T 48T
EUR 503
Description: A sandwich quantitative ELISA assay kit for detection of Goat Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Goat Interleukin 1 Alpha (IL1a) ELISA Kit

DLR-IL1a-g-96T 96T
EUR 654
Description: A sandwich quantitative ELISA assay kit for detection of Goat Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Interleukin 1 Alpha (IL1a) ELISA Kit

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Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Interleukin 1 Alpha (IL1a) ELISA Kit

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Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Interleukin 1 Alpha (IL1a) ELISA Kit

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EUR 435
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Mouse Interleukin 1 Alpha (IL1a) ELISA Kit

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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Interleukin 1 Alpha (IL1a) ELISA Kit

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EUR 493
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Interleukin 1 Alpha (IL1a) ELISA Kit

DLR-IL1a-p-96T 96T
EUR 641
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Interleukin 1 Alpha (IL1a) ELISA Kit

DLR-IL1a-Ra-48T 48T
EUR 323
Description: A sandwich quantitative ELISA assay kit for detection of Rat Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Interleukin 1 Alpha (IL1a) ELISA Kit

DLR-IL1a-Ra-96T 96T
EUR 407
Description: A sandwich quantitative ELISA assay kit for detection of Rat Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rabbit Interleukin 1 Alpha (IL1a) ELISA Kit

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EUR 454
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rabbit Interleukin 1 Alpha (IL1a) ELISA Kit

DLR-IL1a-Rb-96T 96T
EUR 587
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Interleukin 1 Alpha (IL1a) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

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EUR 683

Equine Interleukin 1 Alpha (IL1a) ELISA Kit

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EUR 528

Equine Interleukin 1 Alpha (IL1a) ELISA Kit

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EUR 733

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EUR 528

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EUR 733

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EUR 436

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EUR 601

Mouse Interleukin 1 Alpha (IL1a) ELISA Kit

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EUR 447

Mouse Interleukin 1 Alpha (IL1a) ELISA Kit

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EUR 618

Porcine Interleukin 1 Alpha (IL1a) ELISA Kit

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Porcine Interleukin 1 Alpha (IL1a) ELISA Kit

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EUR 716

Rat Interleukin 1 Alpha (IL1a) ELISA Kit

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EUR 315

Rat Interleukin 1 Alpha (IL1a) ELISA Kit

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EUR 429

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EUR 470

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EUR 651

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Description: A sandwich quantitative ELISA assay kit for detection of Guinea pig Interleukin 1 Alpha (IL1a) in samples from serum, plasma or other biological fluids.

Guinea pig Interleukin 1 Alpha (IL1a) ELISA Kit

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EUR 614
Description: A sandwich quantitative ELISA assay kit for detection of Guinea pig Interleukin 1 Alpha (IL1a) in samples from serum, plasma or other biological fluids.

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Interleukin 1 Alpha (IL1a) Antibody

20-abx128132
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Interleukin 1 Alpha (IL1A) Antibody

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  • EUR 411.00
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  • 100 ug
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Interleukin 1 Alpha (IL1A) Antibody

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  • EUR 732.00
  • EUR 398.00
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  • EUR 411.00
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  • 100 ul
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Interleukin 1 Alpha (IL1a) Antibody

20-abx104904
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
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Interleukin 1 Alpha (IL1a) Antibody

20-abx101885
  • EUR 453.00
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  • 100 ug
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Interleukin 1 Alpha (IL1a) Antibody

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  • EUR 314.00
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  • 100 ug
  • 10 ug
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Interleukin 1 Alpha (IL1a) Antibody

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  • EUR 300.00
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  • EUR 759.00
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Interleukin 1 Alpha (IL1A) Antibody

20-abx101888
  • EUR 425.00
  • EUR 133.00
  • EUR 1177.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Interleukin 1 Alpha (IL1a) Antibody

20-abx101889
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Interleukin 1 Alpha (IL1a) Antibody

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  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
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  • 100 ug
  • 10 ug
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Interleukin 1 Alpha (IL1a) Antibody

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Interleukin 1 Alpha (IL1a) Antibody

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Interleukin 1 Alpha (IL1A) Antibody

abx036412-100ug 100 ug
EUR 391

Interleukin 1 Alpha (IL1a) Antibody

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  • 100 ug
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Interleukin 1 Alpha (IL1a) Antibody

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Interleukin 1 Alpha (IL1a) Antibody

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  • EUR 356.00
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  • 100 ug
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  • 200 ug
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Interleukin 1 Alpha (IL1A) Antibody

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  • 200 ug
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Interleukin 1 Alpha (IL1A) Antibody

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Interleukin 1 Alpha (IL1A) Antibody

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Interleukin 1 Alpha (IL1a) Antibody

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  • EUR 787.00
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  • 100 ug
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Interleukin 1 Alpha (IL1A) Antibody

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EUR 411

Interleukin 1 Alpha (IL1A) Antibody

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Interleukin 1 Alpha (IL1A) Antibody

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Interleukin 1 Alpha (IL1A) Antibody

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Interleukin 1 Alpha (IL1A) Antibody

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Interleukin 1 Alpha (IL1A) Antibody

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  • EUR 411.00
  • EUR 300.00
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Interleukin 1 Alpha (IL1a) Antibody

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  • EUR 815.00
  • EUR 425.00
  • 1 mg
  • 200 ug

Interleukin 1 Alpha (IL1a) Antibody

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  • EUR 453.00
  • EUR 133.00
  • EUR 1288.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
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Interleukin 1 Alpha (IL1a) Antibody

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  • EUR 1372.00
  • EUR 648.00
  • 1 mg
  • 200 ug

Interleukin 1 Alpha (IL1a) Antibody

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  • EUR 871.00
  • EUR 453.00
  • 1 mg
  • 200 ug

Interleukin 1 Alpha (IL1a) Antibody

20-abx173021
  • EUR 871.00
  • EUR 453.00
  • 1 mg
  • 200 ug

Interleukin 1 Alpha (IL1a) Antibody

20-abx173022
  • EUR 913.00
  • EUR 467.00
  • 1 mg
  • 200 ug

Interleukin 1 Alpha (IL1a) Antibody

20-abx173023
  • EUR 913.00
  • EUR 467.00
  • 1 mg
  • 200 ug

Interleukin 1 Alpha (IL1a) Antibody

20-abx173024
  • EUR 954.00
  • EUR 481.00
  • 1 mg
  • 200 ug

Interleukin 1 Alpha (IL1A) Antibody

20-abx300512
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Interleukin-1 Alpha (IL1A) Antibody

20-abx225241
  • EUR 370.00
  • EUR 606.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 50 ul

Interleukin 1 Alpha (IL1A) Antibody

20-abx326469
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

Production of lipopolysaccharide (LPS) -mediated pro-inflammatory cytokines and anti-inflammatory cytokine is suppressed and increased respectively by MAP1889c pretreatment in DC and T cells addition, treatment MAP1889c in M. avium -infected macrophages promoted the growth of intracellular bacteria and IL -10 production. These findings indicate that MAP1889c antimycobacterial modulate the host response and can be a potential virulence factor for MAP infection.

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